https://www.labcanvas.com/blog Guides for scientific figures, presentations, structures and data. en Mon, 19 Oct 2026 09:00:00 GMT https://www.labcanvas.com/blog/imagej-vs-labcanvas https://www.labcanvas.com/blog/imagej-vs-labcanvas Sun, 08 Nov 2026 09:00:00 GMT ImageJ/Fiji or LabCanvas Image Analysis? Six everyday jobs done in both, six newer ones (macros, vendor files, 3D, StarDist, big images) and which to use. ImageJ vs LabCanvas ImageJ alternative ImageJ online Fiji ImageJ count cells online western blot densitometry nanoparticle size distribution colocalization CTCF scale bar ImageJ macro StarDist 3D object analysis deconvolution image stitching whole slide image image analysis software comparison https://www.labcanvas.com/blog/image-analysis-microscopy-western-blot https://www.labcanvas.com/blog/image-analysis-microscopy-western-blot Fri, 06 Nov 2026 09:00:00 GMT How to measure images in LabCanvas Image Analysis: guided assays (nuclear translocation, spots per cell, wound healing, FRAP, calcium, tracking, branching), western blots with a ladder and total-protein normalisation, cell counting with a trainable classifier, ROIs with ImageJ definitions, on the raw pixel values, with results sent to Graphs. image analysis ImageJ alternative Fiji alternative western blot quantification cell counting nuclear translocation wound healing assay FRAP particle tracking microscopy https://www.labcanvas.com/blog/image-analysis-complete-guide https://www.labcanvas.com/blog/image-analysis-complete-guide Fri, 06 Nov 2026 09:00:00 GMT Every tool in LabCanvas Image Analysis, tab by tab, with its settings and a real example: image check, display and scale, stacks and Z projection, background subtraction, filters, thresholds and binary operations, ROIs and Set measurements, Analyze Particles, a pixel classifier, spots, cell counter, western blot densitometry, colocalisation, colour deconvolution and eleven guided assays. image analysis microscopy ROI manager analyze particles western blot quantification colocalisation colour deconvolution Z projection cell counting FRAP particle tracking Sholl analysis https://www.labcanvas.com/blog/how-to-use-imagej https://www.labcanvas.com/blog/how-to-use-imagej Fri, 06 Nov 2026 09:00:00 GMT How to use ImageJ and Fiji: get started, open an image, then 10 everyday jobs (scale, measure, threshold, count, background, blots) with exact menu paths. how to use ImageJ ImageJ tutorial how to use Fiji Fiji tutorial what is ImageJ ImageJ manual image analysis microscopy cell counting western blot quantification ImageJ alternative https://www.labcanvas.com/blog/imagej-scale-bar-set-scale https://www.labcanvas.com/blog/imagej-scale-bar-set-scale Fri, 06 Nov 2026 09:00:00 GMT Set the scale in ImageJ (Analyze › Set Scale) from a micrometer, scale bar or pixel size, add a scale bar, and practise on a free calibration image. how to add scale bar in ImageJ ImageJ scale bar set scale ImageJ calibration image for ImageJ stage micrometer pixels to micrometers pixel size spatial calibration Fiji scale bar https://www.labcanvas.com/blog/western-blot-quantification-imagej https://www.labcanvas.com/blog/western-blot-quantification-imagej Fri, 06 Nov 2026 09:00:00 GMT Quantify a western blot in ImageJ with Analyze › Gels, normalise to a loading control or total protein, and get fold change. Plus a 3-click way. western blot quantification ImageJ western blot quantification western blot analysis ImageJ densitometry ImageJ ImageJ gel analysis band intensity loading control total protein normalisation relative density fold change Fiji https://www.labcanvas.com/blog/count-cells-imagej https://www.labcanvas.com/blog/count-cells-imagej Fri, 06 Nov 2026 09:00:00 GMT Count cells in ImageJ: automatic (threshold, Watershed, Analyze Particles) or manual (Cell Counter, Multi-point), plus cells per mm² and % positive. ImageJ cell counting how to count cells in ImageJ Cell Counter plugin automated cell counting count nuclei Analyze Particles threshold watershed Fiji cells per mm² percent positive https://www.labcanvas.com/blog/measure-length-area-imagej https://www.labcanvas.com/blog/measure-length-area-imagej Fri, 06 Nov 2026 09:00:00 GMT Measure length and area in ImageJ: set the scale, draw lines or area selections (polygon, wand, threshold), press Measure, and get µm and µm², not pixels. ImageJ length measurement how to use ImageJ to measure length ImageJ area measurement how to measure area in ImageJ ImageJ measurements Set Measurements segmented line wand tool pixels to µm Fiji https://www.labcanvas.com/blog/grain-size-analysis-sem https://www.labcanvas.com/blog/grain-size-analysis-sem Fri, 06 Nov 2026 09:00:00 GMT Measure grain size from SEM images in ImageJ: closed boundaries, Analyze Particles, then ASTM E112 G from mean area or intercept, with a worked example. ImageJ grain size grain size analysis ImageJ ASTM E112 grain size number calculator ASTM E112 calculator intercept method grain size planimetric method SEM image analysis thin film grain size perovskite film analyze particles equivalent diameter https://www.labcanvas.com/blog/leaf-area-imagej https://www.labcanvas.com/blog/leaf-area-imagej Fri, 06 Nov 2026 09:00:00 GMT Measure leaf area in ImageJ from a photo or scan: set the scale from a ruler, threshold the leaf, run Analyze Particles in cm², with or without holes. ImageJ leaf area leaf area ImageJ how to measure leaf area from a photo leaf area measurement ImageJ leaf Analyze Particles Color Threshold insect damage set scale from a ruler Fiji https://www.labcanvas.com/blog/imagej-online https://www.labcanvas.com/blog/imagej-online Fri, 06 Nov 2026 09:00:00 GMT Yes: ImageJ.JS runs ImageJ 1.x in your browser via CheerpJ (WebAssembly). What works, what doesn't, Fiji online, iPad and Android, and a faster option. ImageJ online ImageJ.JS ImageJ JavaScript ImageJ JS ImageJ browser ImageJ web version Fiji online ImageJ iPad ImageJ Android CheerpJ ImJoy ImageJ alternative https://www.labcanvas.com/blog/imagej-alternatives https://www.labcanvas.com/blog/imagej-alternatives Fri, 06 Nov 2026 09:00:00 GMT The best ImageJ alternatives compared: browser tools, Fiji, CellProfiler, QuPath, ilastik, napari, Icy, MIPAR, and Image Lab and Empiria for blots. ImageJ alternative ImageJ alternatives Fiji alternative software like ImageJ best image analysis software free image analysis software microscopy image analysis CellProfiler QuPath ilastik napari western blot software https://www.labcanvas.com/blog/2d-flake-lateral-size https://www.labcanvas.com/blog/2d-flake-lateral-size Fri, 06 Nov 2026 09:00:00 GMT Measure graphene or MoS₂ flake lateral size in ImageJ: scale, threshold, Analyze Particles with Feret diameter, edge flakes, overlaps and a size histogram. flake size distribution graphene flake size SEM lateral size MoS2 2D material flake size ImageJ Feret diameter Analyze Particles nanosheet size SEM image analysis TEM image analysis size histogram ImageJ https://www.labcanvas.com/blog/analyze-particles-imagej https://www.labcanvas.com/blog/analyze-particles-imagej Fri, 06 Nov 2026 09:00:00 GMT Every Analyze Particles option in ImageJ, shown on a TEM image: size, circularity, Show, Exclude on edges, Include holes, and why it finds 0 or 1 particle. analyze particles ImageJ ImageJ particle analysis count particles ImageJ ImageJ particle size analysis size distribution ImageJ exclude on edges include holes circularity TEM nanoparticles watershed Fiji https://www.labcanvas.com/blog/calcium-imaging-delta-f-over-f https://www.labcanvas.com/blog/calcium-imaging-delta-f-over-f Fri, 06 Nov 2026 09:00:00 GMT Calcium imaging in ImageJ: ROIs on cells, Multi Measure, background, F₀ (first frames or a percentile), ΔF/F₀ = (F − F₀)/F₀, traces and peak counting. calcium imaging analysis imagej delta f over f calculation df/f0 ΔF/F₀ GCaMP calcium transients Plot Z-axis Profile Multi Measure baseline F0 peak detection ROI Manager https://www.labcanvas.com/blog/cellprofiler-vs-qupath-vs-imagej https://www.labcanvas.com/blog/cellprofiler-vs-qupath-vs-imagej Fri, 06 Nov 2026 09:00:00 GMT CellProfiler, QuPath or ImageJ/Fiji? A fair comparison by job (batch cell measurements, whole slides, general analysis), with a nuclei count step by step. CellProfiler vs ImageJ QuPath vs ImageJ best cell image analysis software CellProfiler QuPath Fiji ImageJ nuclei counting digital pathology high-content screening bioimage analysis software https://www.labcanvas.com/blog/colocalization-analysis-pearson-manders https://www.labcanvas.com/blog/colocalization-analysis-pearson-manders Fri, 06 Nov 2026 09:00:00 GMT Colocalization in ImageJ and Fiji: what Pearson’s r and Manders’ M1/M2 each answer, Costes thresholds, Coloc 2 vs JACoP, a worked example and pitfalls. colocalization ImageJ ImageJ colocalization JACoP ImageJ Coloc 2 colocalization plugin Pearson vs Manders Pearson's correlation coefficient Manders' coefficients Costes threshold Fiji fluorescence microscopy https://www.labcanvas.com/blog/colony-counter-online https://www.labcanvas.com/blog/colony-counter-online Fri, 06 Nov 2026 09:00:00 GMT Count colonies in ImageJ: oval on the dish, threshold, Watershed, Analyze Particles. Then CFU/mL from the count and dilution, with a worked example. ImageJ colony counting colony counter ImageJ count colonies ImageJ colony counter online CFU counter CFU/mL calculation plate count Analyze Particles watershed Fiji https://www.labcanvas.com/blog/contact-angle-imagej https://www.labcanvas.com/blog/contact-angle-imagej Fri, 06 Nov 2026 09:00:00 GMT Measure contact angle in ImageJ with the Angle tool along the tangent, or from drop height and base width: θ = 2·arctan(2h/d). Worked example, plugins. contact angle ImageJ ImageJ contact angle plugin drop analysis ImageJ sessile drop DropSnake LB-ADSA spherical cap wettability Bond number surface energy https://www.labcanvas.com/blog/count-foci-per-nucleus https://www.labcanvas.com/blog/count-foci-per-nucleus Fri, 06 Nov 2026 09:00:00 GMT Count γH2AX or 53BP1 foci per nucleus in ImageJ: nuclei as ROIs, Find Maxima with the right prominence, counts per nucleus and % of cells with ≥5 foci. count foci ImageJ gamma H2AX foci quantification DNA damage foci counting 53BP1 foci Find Maxima prominence foci per nucleus ROI Manager Fiji puncta counting https://www.labcanvas.com/blog/d-spacing-hrtem-lattice-fringes https://www.labcanvas.com/blog/d-spacing-hrtem-lattice-fringes Fri, 06 Nov 2026 09:00:00 GMT Measure d-spacing in ImageJ: a wide line across 10 fringes and Plot Profile (d = L ÷ N), or FFT spots read with Measure. Worked on Au (111), 0.235 nm. d spacing imagej lattice fringe measurement d spacing hrtem interplanar spacing tem fft imagej HRTEM lattice spacing power spectrum Plot Profile gold nanoparticles Fiji https://www.labcanvas.com/blog/dab-ihc-quantification https://www.labcanvas.com/blog/dab-ihc-quantification Fri, 06 Nov 2026 09:00:00 GMT Quantify DAB IHC in ImageJ/Fiji: colour deconvolution with H-DAB vectors, % positive area, optical density, and why DAB intensity is not linear. color deconvolution ImageJ IHC quantification ImageJ DAB quantification ImageJ immunohistochemistry colour deconvolution H-DAB optical density percent positive area Fiji Color Threshold https://www.labcanvas.com/blog/feret-diameter-circularity https://www.labcanvas.com/blog/feret-diameter-circularity Fri, 06 Nov 2026 09:00:00 GMT What ImageJ shape descriptors mean: Feret and MinFeret, circularity, AR, roundness and solidity, with formulas, drawn shapes and real TEM and SEM numbers. feret diameter ImageJ circularity ImageJ solidity ImageJ shape descriptors ImageJ aspect ratio roundness MinFeret convex hull nanoparticle shape nanorod aspect ratio particle shape analysis https://www.labcanvas.com/blog/fiji-stardist-cellpose-segmentation https://www.labcanvas.com/blog/fiji-stardist-cellpose-segmentation Fri, 06 Nov 2026 09:00:00 GMT When a threshold fails, segment cells in Fiji with StarDist, Cellpose or Trainable Weka: install, settings, then label image to ROIs and measurements. StarDist ImageJ Cellpose ImageJ ImageJ deep learning Trainable Weka Segmentation deepImageJ ImageJ segmentation plugin Fiji label image nuclei segmentation touching cells ROI Manager https://www.labcanvas.com/blog/fiji-stitching-tiles https://www.labcanvas.com/blog/fiji-stitching-tiles Fri, 06 Nov 2026 09:00:00 GMT Stitch overlapping microscope tiles in Fiji with Grid/Collection stitching: grid type, overlap, file names, fusion, and why an ImageJ montage is wrong. ImageJ stitching stitch images ImageJ image stitching ImageJ Fiji Grid/Collection stitching tile scan mosaic tile overlap TileConfiguration Make Montage phase correlation https://www.labcanvas.com/blog/fiji-vs-imagej https://www.labcanvas.com/blog/fiji-vs-imagej Fri, 06 Nov 2026 09:00:00 GMT Fiji is ImageJ with plugins, Bio-Formats, an updater and scripting built in; ImageJ2 is the rewrite underneath. A comparison table and which to download. imagej vs fiji fiji vs imagej fiji is just imagej imagej2 what is fiji imagej what is imagej called now ImageJ download Fiji download Bio-Formats ImageJ.JS https://www.labcanvas.com/blog/frap-analysis-mobile-fraction https://www.labcanvas.com/blog/frap-analysis-mobile-fraction Fri, 06 Nov 2026 09:00:00 GMT FRAP in ImageJ: bleach, reference and background ROIs, Multi Measure, double normalisation, an exponential recovery fit, then the mobile fraction and t½. frap analysis imagej frap mobile fraction calculation frap half time double normalization frap FRAP fluorescence recovery after photobleaching ROI Manager Multi Measure Curve Fitting exponential recovery immobile fraction https://www.labcanvas.com/blog/free-western-blot-quantification-software https://www.labcanvas.com/blog/free-western-blot-quantification-software Fri, 06 Nov 2026 09:00:00 GMT Image Studio Lite is discontinued. Quantify western blots free in ImageJ (Analyze › Gels), step by step, with Image Lab and IOCBIO Gel compared. Image Studio Lite alternative free western blot analysis software western blot densitometry software free western blot quantification ImageJ gel analysis Image Lab IOCBIO Gel Empiria Studio densitometry fold change https://www.labcanvas.com/blog/how-to-cite-imagej https://www.labcanvas.com/blog/how-to-cite-imagej Fri, 06 Nov 2026 09:00:00 GMT Cite ImageJ (Schneider et al. 2012), Fiji (Schindelin et al. 2012) or ImageJ2 (Rueden et al. 2017): DOIs, APA, Vancouver, BibTeX and methods sentences. ImageJ citation ImageJ software citation how to cite ImageJ ImageJ reference how to cite ImageJ in methods cite Fiji ImageJ2 methods section BibTeX image analysis https://www.labcanvas.com/blog/imagej-3d-projection https://www.labcanvas.com/blog/imagej-3d-projection Fri, 06 Nov 2026 09:00:00 GMT Make a rotating 3D view of a Z-stack with Image › Stacks › 3D Project in ImageJ, set the slice spacing, use Orthogonal Views, or Fiji's 3D Viewer. 3d viewer imagej imagej 3d viewer 3d reconstruction imagej imagej 3d reconstruction 3D Project Z-stack voxel depth Orthogonal Views Fiji 3D Viewer volume rendering https://www.labcanvas.com/blog/imagej-basic-image-editing https://www.labcanvas.com/blog/imagej-basic-image-editing Fri, 06 Nov 2026 09:00:00 GMT How to crop, rotate, resize, zoom and convert to 8-bit or grayscale in ImageJ, and what each command does to pixel values and the scale, measured. how to crop in imagej how to rotate image in imagej imagej resize image imagej convert to grayscale how to merge images in imagej how to zoom in on imagej Image Crop Image Scale 8-bit conversion pixel size Fiji https://www.labcanvas.com/blog/imagej-deconvolution https://www.labcanvas.com/blog/imagej-deconvolution Fri, 06 Nov 2026 09:00:00 GMT What deconvolution does (the PSF, Richardson–Lucy), why FD Math Deconvolve fails on real images, and how to run Richardson–Lucy in ImageJ and Fiji. deconvolution ImageJ ImageJ deconvolution deconvolution in ImageJ Richardson-Lucy point spread function PSF FD Math DeconvolutionLab2 Iterative Deconvolve 3D Fiji fluorescence microscopy https://www.labcanvas.com/blog/imagej-fft-bandpass-edge-detection https://www.labcanvas.com/blog/imagej-fft-bandpass-edge-detection Fri, 06 Nov 2026 09:00:00 GMT FFT in ImageJ step by step: Process › FFT, Bandpass Filter settings, stripe removal and Find Edges, with before/after pictures and when filtering is safe. fft imagej imagej fft edge detection imagej imagej edge detection FFT bandpass filter Find Edges Sobel power spectrum remove stripes uneven background segmentation https://www.labcanvas.com/blog/imagej-histogram-saturation https://www.labcanvas.com/blog/imagej-histogram-saturation Fri, 06 Nov 2026 09:00:00 GMT Check saturation in ImageJ with Analyze › Histogram, the HiLo LUT and Min/Max, on a clipped example with its spike at 65535 and what it does to data. ImageJ histogram histogram ImageJ saturated pixels check saturation HiLo LUT overexposed clipping Analyze Histogram min and max gray value fluorescence quantification image integrity https://www.labcanvas.com/blog/imagej-lut https://www.labcanvas.com/blog/imagej-lut Fri, 06 Nov 2026 09:00:00 GMT ImageJ lookup tables compared on one image: Grays, Fire, viridis, HiLo and the rest, how to add a calibration bar, and colour-blind-safe channel colours. ImageJ LUT ImageJ LUTs lookup tables ImageJ LUT ImageJ calibration bar Fire LUT viridis HiLo colour blind green magenta false colour https://www.labcanvas.com/blog/imagej-mac https://www.labcanvas.com/blog/imagej-mac Fri, 06 Nov 2026 09:00:00 GMT Download ImageJ or Fiji for Mac (Apple Silicon or Intel), fix "can't be opened" and "damaged" errors, set the memory, and the no-install option. download ImageJ for Mac ImageJ for Mac ImageJ Mac download Fiji download Mac ImageJ for Mac M1 how to use ImageJ on Mac Apple Silicon Gatekeeper Fiji ImageJ memory https://www.labcanvas.com/blog/imagej-macros-batch-processing https://www.labcanvas.com/blog/imagej-macros-batch-processing Fri, 06 Nov 2026 09:00:00 GMT Record an ImageJ macro with Plugins › Macros › Record, run it from the editor, then batch-process a folder with Process › Batch › Macro. Step by step. imagej macro imagej macros macro language imagej imagej macro recorder imagej batch processing batch processing imagej Process Batch Macro Analyze Particles Fiji macro automate ImageJ https://www.labcanvas.com/blog/imagej-make-movie-from-stack https://www.labcanvas.com/blog/imagej-make-movie-from-stack Fri, 06 Nov 2026 09:00:00 GMT Turn a folder of frames or a time-lapse stack into an AVI, GIF or MP4 in ImageJ: Image Sequence, a time stamp, a scale bar, frame rate, and saving. imagej make video from stack imagej save as mp4 mp4 imagej imagej image sequence imagej avi animated gif imagej time stamp time-lapse movie frame rate Fiji https://www.labcanvas.com/blog/imagej-plot-profile https://www.labcanvas.com/blog/imagej-plot-profile Fri, 06 Nov 2026 09:00:00 GMT Plot Profile in ImageJ: draw a line, press Ctrl K, set the line width to average noise, copy the values, plot two channels and profile a rectangle. ImageJ plot profile plot profile ImageJ line scan ImageJ intensity profile line profile line width multichannel profile Fiji colocalisation line scan lattice fringes https://www.labcanvas.com/blog/imagej-roi-manager https://www.labcanvas.com/blog/imagej-roi-manager Fri, 06 Nov 2026 09:00:00 GMT Open the ROI Manager in ImageJ (Analyze › Tools › ROI Manager), add ROIs with T, measure them on every channel, save RoiSet.zip and reopen it later. ROI Manager ImageJ ImageJ ROI how to open ROI Manager ImageJ save ROI select multiple ROI RoiSet.zip Multi Measure measure two channels Fiji regions of interest https://www.labcanvas.com/blog/imagej-threshold-methods https://www.labcanvas.com/blog/imagej-threshold-methods Fri, 06 Nov 2026 09:00:00 GMT All 16 ImageJ auto threshold methods on one image, with the threshold and % area each gives, which method suits which image, and how to apply it. threshold ImageJ ImageJ thresholding ImageJ auto threshold methods which threshold method Otsu vs Triangle segmentation ImageJ setAutoThreshold Convert to Mask dark background Fiji Auto Threshold image segmentation https://www.labcanvas.com/blog/imagej-windows https://www.labcanvas.com/blog/imagej-windows Fri, 06 Nov 2026 09:00:00 GMT Download and install Fiji or ImageJ on Windows 10 and 11: the right zip, where to unzip it (not Program Files), Java, memory, and where files go. ImageJ download Windows Fiji download Windows ImageJ for Windows install Fiji Windows 11 ImageJ Windows 10 ImageJ memory Program Files Fiji Java https://www.labcanvas.com/blog/kymograph-imagej https://www.labcanvas.com/blog/kymograph-imagej Fri, 06 Nov 2026 09:00:00 GMT Make a kymograph in ImageJ: a line along the track, Image › Stacks › Reslice, then a line on each streak gives velocity = Δx ÷ Δt, with a worked example. ImageJ kymograph kymograph ImageJ how to make a kymograph velocity from kymograph Reslice KymoResliceWide KymographBuilder axonal transport time-lapse Fiji https://www.labcanvas.com/blog/maximum-intensity-projection https://www.labcanvas.com/blog/maximum-intensity-projection Fri, 06 Nov 2026 09:00:00 GMT Image › Stacks › Z Project in ImageJ: what Max Intensity, Average, Sum Slices, SD, Min and Median give, when Sum beats Max, and how to check depth. Z project ImageJ maximum intensity projection ImageJ Z projection stack projection sum slices average intensity projection orthogonal views Z-stack confocal Fiji https://www.labcanvas.com/blog/measure-fluorescence-intensity-imagej https://www.labcanvas.com/blog/measure-fluorescence-intensity-imagej Fri, 06 Nov 2026 09:00:00 GMT Measure fluorescence in ImageJ: Mean, IntDen and RawIntDen explained, CTCF = IntDen − Area × background worked through, and the 8-bit and saturation traps. integrated density ImageJ how to measure fluorescence intensity in ImageJ what is integrated density mean gray value CTCF corrected total cell fluorescence RawIntDen quantify fluorescence intensity ImageJ background subtraction Set Measurements Fiji https://www.labcanvas.com/blog/merge-channels-imagej https://www.labcanvas.com/blog/merge-channels-imagej Fri, 06 Nov 2026 09:00:00 GMT Merge channels in ImageJ with Image › Color › Merge Channels, split them with Split Channels, overlay with Add Image, and build a colour-blind-safe figure. ImageJ merge channels overlay ImageJ how to split channels in ImageJ how to merge images in ImageJ composite image Channels Tool Stack to RGB green magenta colour-blind safe fluorescence figure Fiji https://www.labcanvas.com/blog/microscopy-image-integrity https://www.labcanvas.com/blog/microscopy-image-integrity Fri, 06 Nov 2026 09:00:00 GMT What journals allow when you adjust microscopy and blot images: linear whole-image changes, gamma disclosed, no cloning or selective edits, in ImageJ. image manipulation guidelines microscopy western blot image integrity journal image guidelines image integrity brightness and contrast gamma adjustment research integrity blot splicing ImageJ figure preparation https://www.labcanvas.com/blog/nanoparticle-size-distribution-tem https://www.labcanvas.com/blog/nanoparticle-size-distribution-tem Fri, 06 Nov 2026 09:00:00 GMT Nanoparticle size from TEM in ImageJ: scale, threshold, watershed, Analyze Particles with Feret, then the size histogram and mean ± SD, worked through. nanoparticle size distribution ImageJ TEM particle size analysis how to measure particle size in ImageJ particle size histogram TEM Feret diameter equivalent diameter gold nanoparticles watershed analyze particles TEM image analysis https://www.labcanvas.com/blog/nanowire-length-aspect-ratio-sem https://www.labcanvas.com/blog/nanowire-length-aspect-ratio-sem Fri, 06 Nov 2026 09:00:00 GMT Measure nanorod and nanowire length, width and aspect ratio from SEM images in ImageJ: Feret, MinFeret, fit ellipse, edge rods, and why not to watershed. nanowire length ImageJ nanorod aspect ratio measure nanowire diameter SEM Feret diameter MinFeret aspect ratio ImageJ SEM image analysis Analyze Particles nanorod size distribution Fiji https://www.labcanvas.com/blog/neurite-length-skeleton-analysis https://www.labcanvas.com/blog/neurite-length-skeleton-analysis Fri, 06 Nov 2026 09:00:00 GMT Neurite length and branching in ImageJ: threshold, Process › Binary › Skeletonize, then Analyze Skeleton (Fiji) or a macro. Why diagonal steps matter. skeletonize imagej imagej skeletonize analyze skeleton neurite length imagej branch length neurite outgrowth skeleton analysis branch points Fiji AnalyzeSkeleton https://www.labcanvas.com/blog/nuclear-cytoplasmic-ratio https://www.labcanvas.com/blog/nuclear-cytoplasmic-ratio Fri, 06 Nov 2026 09:00:00 GMT Measure the nuclear/cytoplasmic ratio in ImageJ step by step: nuclei from DAPI, a cytoplasm ring with Enlarge and Make Band, and an N/C table per cell. nuclear cytoplasmic ratio ImageJ NF-kB translocation quantification YAP nuclear localization quantification TFEB nuclear translocation N/C ratio Make Band Enlarge selection ROI Manager cytoplasmic ring Fiji https://www.labcanvas.com/blog/open-czi-nd2-files-imagej https://www.labcanvas.com/blog/open-czi-nd2-files-imagej Fri, 06 Nov 2026 09:00:00 GMT Open Zeiss .czi, Nikon .nd2 and Leica .lif files in ImageJ with Bio-Formats (built into Fiji), keep the pixel size, and fix "Unsupported format". Bio-Formats ImageJ how to open czi files in ImageJ how to open nd2 files in ImageJ ImageJ Bio-Formats plugin lif files ImageJ OME-TIFF Fiji pixel size microscope file formats Unsupported format or file not found https://www.labcanvas.com/blog/open-dicom-imagej https://www.labcanvas.com/blog/open-dicom-imagej Fri, 06 Nov 2026 09:00:00 GMT Open DICOM files in ImageJ with File › Open, a whole series as a stack with Import › Image Sequence, read the tags, measure HU, and open compressed DICOM. ImageJ DICOM compressed DICOM ImageJ is ImageJ good for medical imaging open DICOM series DICOM stack Hounsfield units Show Info Bio-Formats CT Fiji https://www.labcanvas.com/blog/particle-tracking-msd https://www.labcanvas.com/blog/particle-tracking-msd Fri, 06 Nov 2026 09:00:00 GMT Track particles in ImageJ with TrackMate (Fiji) or Find Maxima, then get the diffusion coefficient from the MSD: MSD = 4Dt in 2D, with a worked example. particle tracker ImageJ particle tracking ImageJ TrackMate ImageJ MSD analysis mean squared displacement diffusion coefficient from tracking single-particle tracking Find Maxima Fiji drift correction https://www.labcanvas.com/blog/porosity-pore-size-sem-image https://www.labcanvas.com/blog/porosity-pore-size-sem-image Fri, 06 Nov 2026 09:00:00 GMT Porosity and pore size from an SEM image in ImageJ: threshold the pores, read %Area as porosity, then Analyze Particles for pore areas and diameters. ImageJ porosity porosity from SEM image pore size distribution SEM ImageJ measure pore size SEM area fraction ImageJ anodic aluminium oxide AAO membrane porous coating equivalent diameter analyze particles SEM image analysis https://www.labcanvas.com/blog/pyimagej-python https://www.labcanvas.com/blog/pyimagej-python Fri, 06 Nov 2026 09:00:00 GMT Install PyImageJ, start ImageJ from Python with imagej.init, run your ImageJ macro, and get the image as NumPy and the Results table as pandas. PyImageJ ImageJ Python imagej.init run ImageJ macro from Python ImageJ NumPy ImageJ pandas Fiji Python ImageJ2 batch processing Jupyter https://www.labcanvas.com/blog/rolling-ball-background-subtraction https://www.labcanvas.com/blog/rolling-ball-background-subtraction Fri, 06 Nov 2026 09:00:00 GMT Subtract background in ImageJ: Process › Subtract Background, a rolling ball radius at least the largest object's, Light background off for fluorescence. subtract background ImageJ ImageJ subtract background background subtraction ImageJ rolling ball radius rolling ball algorithm sliding paraboloid uneven illumination Fiji fluorescence microscopy line profile https://www.labcanvas.com/blog/scratch-wound-healing-assay-analysis https://www.labcanvas.com/blog/scratch-wound-healing-assay-analysis Fri, 06 Nov 2026 09:00:00 GMT Measure a scratch wound in ImageJ: variance filter, threshold and Analyze Particles per frame, then % closure and closure rate, with a worked example. wound healing assay imagej scratch assay analysis wound closure percentage calculation wound healing size tool imagej scratch assay migration assay variance filter closure rate time-lapse ImageJ https://www.labcanvas.com/blog/sholl-analysis https://www.labcanvas.com/blog/sholl-analysis Fri, 06 Nov 2026 09:00:00 GMT Sholl analysis step by step: circles around the cell body, crossings per radius, the Sholl plot, critical radius and ramification index, in Fiji (SNT) or with an ImageJ macro. sholl analysis imagej sholl analysis online sholl analysis fiji dendritic complexity dendritic arborization ramification index critical radius neuron morphology SNT Sholl plot https://www.labcanvas.com/blog/tube-formation-assay-quantification https://www.labcanvas.com/blog/tube-formation-assay-quantification Fri, 06 Nov 2026 09:00:00 GMT Quantify a tube formation assay in ImageJ step by step: total tube length, junctions, segments and meshes, plus the Angiogenesis Analyzer and AngioTool. tube formation assay quantification Angiogenesis Analyzer ImageJ AngioTool endothelial tube formation analysis HUVEC tube formation skeletonize junctions and meshes total tube length angiogenesis assay Fiji https://www.labcanvas.com/blog/watershed-touching-particles https://www.labcanvas.com/blog/watershed-touching-particles Fri, 06 Nov 2026 09:00:00 GMT Split touching nuclei and nanoparticles with Process › Binary › Watershed in ImageJ, with before and after counts, and why it cuts nanorods to pieces. ImageJ watershed watershed ImageJ separate touching particles split nuclei touching cells distance map ultimate points binary watershed over-segmentation Analyze Particles nanoparticle counting https://www.labcanvas.com/blog/crystal-structure-visualization-software https://www.labcanvas.com/blog/crystal-structure-visualization-software Mon, 26 Oct 2026 09:00:00 GMT Which tool for crystal structure and 2D-material figures? VESTA, CrystalMaker, Mercury, Avogadro, Jmol, OVITO, the Materials Project viewer and LabCanvas compared: licence, platforms, file formats, rendering and how to cite each. crystal structure VESTA CIF 2D materials materials science figures comparison https://www.labcanvas.com/blog/free-tools-scientific-figures https://www.labcanvas.com/blog/free-tools-scientific-figures Mon, 26 Oct 2026 09:00:00 GMT An honest comparison of tools for scientific figures: BioRender, Mind the Graph, Inkscape, Affinity, Canva, PowerPoint, Adobe Illustrator and LabCanvas. 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LabCanvas MolDraw: atoms, bonds, rings and charges, reaction arrows, structures from PubChem, SMILES, a 3D view of the same molecule, and placing structures in figures. chemical structure drawing molecule editor reaction scheme ChemDraw alternative SMILES chemistry https://www.labcanvas.com/blog/3d-studio-scientific-3d-models https://www.labcanvas.com/blog/3d-studio-scientific-3d-models Sun, 18 Oct 2026 09:00:00 GMT How to make 3D scientific renders in the LabCanvas 3D Studio: adjustable models of cells, viruses, nanoparticles, 2D materials, MOFs and devices, molecules from PubChem, proteins from the PDB, crystals from CIF files, and renders placed in figures. 3D scientific illustration nanomaterials 3D cell 3D model crystal structure MOF ray tracing https://www.labcanvas.com/blog/protein-viewer-pdb-structures https://www.labcanvas.com/blog/protein-viewer-pdb-structures Sun, 18 Oct 2026 09:00:00 GMT How to show a protein structure in the LabCanvas Protein viewer: load any PDB ID or your own PDB and mmCIF file, choose cartoon, surface or ball-and-stick, colour by chain or secondary structure, select residues on the sequence and snapshot a transparent PNG for your figure. protein structure PDB viewer molecular visualization structural biology AlphaFold PyMOL alternative https://www.labcanvas.com/blog/graphs-statistics-scientific-plots https://www.labcanvas.com/blog/graphs-statistics-scientific-plots Sun, 18 Oct 2026 09:00:00 GMT A visual guide to LabCanvas Graphs & statistics: Prism-style data tables, 70 graph types from scatter dot plots to Kaplan–Meier, volcano and PAE plots, t tests, ANOVA, curve fits and survival tests checked against SciPy, and graphs that stay linked to their figure. scientific graphs statistics GraphPad Prism alternative ANOVA Kaplan-Meier volcano plot dose-response curve https://www.labcanvas.com/blog/optics-optical-setup-simulator https://www.labcanvas.com/blog/optics-optical-setup-simulator Sun, 18 Oct 2026 09:00:00 GMT How to build optical setup diagrams in LabCanvas Optics: lasers, lenses, mirrors, beam splitters, gratings and detectors on a breadboard, rays and beams traced as you move parts, interference, ready-made setups and clean schematics for figures. optical setup diagram ray tracing optics simulation laser setup interferometer physics illustration https://www.labcanvas.com/blog/pixel-editor-microscopy-images https://www.labcanvas.com/blog/pixel-editor-microscopy-images Sun, 18 Oct 2026 09:00:00 GMT How to adjust microscopy images and photos in the LabCanvas Pixel editor: crop, levels, curves and exposure, false-colour lookup tables, healing and clone stamp, selections and masks, with an edit log for your methods section. microscopy image editing image processing figure preparation lookup tables image integrity Photoshop alternative https://www.labcanvas.com/blog/what-is-labcanvas https://www.labcanvas.com/blog/what-is-labcanvas Wed, 14 Oct 2026 09:00:00 GMT A guide to LabCanvas: ten connected apps for figures, molecules, 3D crystals, graphs, slides and posters, the workflows between them, ready-made templates, and why one editable canvas makes scientific illustration faster to learn and easier to change. 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